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pet30a/txgh116δ1-18 expression vector  (Agilent technologies)


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    Structured Review

    Agilent technologies pet30a/txgh116δ1-18 expression vector
    Pet30a/Txgh116δ1 18 Expression Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet30a+expression+vector/pm37180965-152-19-28
    Average 90 stars, based on 1 article reviews
    pet30a/txgh116δ1-18 expression vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Novel centrosome protein, TCC52, is a cancer‐testis antigen
    Article Snippet: .. The PCR product was digested with EcoRI and HindIII and cloned into the expression vector pET30a (Stratagene, La Jolla, CA, USA). .. TCC52 recombinant protein fused with two 6His‐tags was purified by Ni +2 affinity chromatography by using the NiNTA agarose resin (Invitrogen).

    Clone Assay:

    Article Title: Novel centrosome protein, TCC52, is a cancer‐testis antigen
    Article Snippet: .. The PCR product was digested with EcoRI and HindIII and cloned into the expression vector pET30a (Stratagene, La Jolla, CA, USA). .. TCC52 recombinant protein fused with two 6His‐tags was purified by Ni +2 affinity chromatography by using the NiNTA agarose resin (Invitrogen).

    Expressing:

    Article Title: Novel centrosome protein, TCC52, is a cancer‐testis antigen
    Article Snippet: .. The PCR product was digested with EcoRI and HindIII and cloned into the expression vector pET30a (Stratagene, La Jolla, CA, USA). .. TCC52 recombinant protein fused with two 6His‐tags was purified by Ni +2 affinity chromatography by using the NiNTA agarose resin (Invitrogen).

    Plasmid Preparation:

    Article Title: Novel centrosome protein, TCC52, is a cancer‐testis antigen
    Article Snippet: .. The PCR product was digested with EcoRI and HindIII and cloned into the expression vector pET30a (Stratagene, La Jolla, CA, USA). .. TCC52 recombinant protein fused with two 6His‐tags was purified by Ni +2 affinity chromatography by using the NiNTA agarose resin (Invitrogen).

    other:

    Article Title: Reaction Mechanism of Glycoside Hydrolase Family 116 Utilizes Perpendicular Protonation.
    Article Snippet: The TxGH116 D593A mutation has been described previously.8 The mutations D593E, D593G, and D593N were also made in the pET30a/TxGH116Δ1-18 expression vector by the QuikChange Site-directed mutagenesis method (Stratagene, Agilent Corp.).

    Article Title: New insights into RNA packaging in porcine reproductive and respiratory syndrome virus.
    Article Snippet: Therefore, a 59His–N fusion protein expression vector (pET30a-59His-N) was constructed by using conventional cloning and was transformed into BL21-Gold (DE3) pLysS cells (Stratagene).



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